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Correlation analysis and canonical correlation analysis between flavonoids content and expression of synthesis-related genes Yu, F. Q., Xu, X. Y., Lin, S., Peng, T., Zeng, S. H. (2022). Integrated metabolomics and transcriptomics reveal flavonoids glycosylation difference in two citrus peels. Scientia Hortic. 292. doi:10.1016/j.scienta.2021.110623 Environmental factors can regulate gene expression by influencing TFs, which bind specifically to the promoters of their target genes. Among TF families, the MYB family has been shown to play a critical role in regulating gene expression in the flavonoid pathway ( Espley etal., 2007; Zhou etal., 2015; Zhai etal., 2016; Li etal., 2020a). For instance, the MdBBX22–miR858–MdMYB9/11/12 was found to activate the promoters of MdANR and MdLAR in apple, thereby promoting the biosynthesis of proanthocyanidin ( Zhang etal., 2022). In this study, one CitMYB (Cs_ont_1g021030) was identified as highly related to structural genes and seven flavonoids based on WGCNA. Therefore, these ten TF genes were considered important in regulating the flavonoid content of SOPs. Although the results of qRT-PCR showed good consistency with the transcriptome data ( Supplementary Figure10), future studies are needed to elucidate the function of these genes in flavonoid biosynthesis. Conclusion Rowan, D. D., Cao, M., Lin-Wang, K., Cooney, J. M., Jensen, D. J., Austin, P. T., et al. (2009). Environmental regulation of leaf colour in red 35S:PAP1 arabidopsis thaliana. New Phytol. 182, 102–115. doi:10.1111/j.1469-8137.2008.02737.x Mahmoud, A. M., Hernandez Bautista, R. J., Sandhu, M. A., Hussein, O. E. (2019). Beneficial effects of citrus flavonoids on cardiovascular and metabolic health. Oxid. Med. Cell Longev 2019, 5484138. doi:10.1155/2019/5484138
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Bo Xiong *† Qin Li † Junfei Yao Zhuyuan Liu Xinxia Yang Xiaoyong Yu 1 Yuan Li Ling Liao Xun Wang Honghong Deng Mingfei Zhang Guochao Sun Zhihui Wang *
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Peng, Y., Hu, M. J., Lu, Q., Tian, Y., He, W. Y., Chen, L., et al. (2019). Flavonoids derived from exocarpium citri grandis inhibit LPS-induced inflammatory response via suppressing MAPK and NF-kappa b signalling pathways. Food Agric. Immunol. 30, 564–580. doi:10.1080/09540105.2018.1550056 The samples underwent a series of preparation steps, including immersion in liquid nitrogen, grinding to a fine powder, suspension in 70% methanol and centrifugation at 20,000 rpm for 20 minutes at 4°C. The supernatant was then filtered through a 0.22-μm nylon syringe filter before being subjected to UPLC-MS analysis. This involved the use of an Agilent SB-C18 column (2.1 × 100 mm, 1.8 μm), with mobile phase A consisting of water with 0.1% formic acid, while mobile phase B was a solution of 0.1% formic acid in acetonitrile. The elution procedure utilized a gradient of 0-95% B from 0-9 min, followed by a single minute at 95% B and further gradient steps of 95-5% B for 1 minute and 5% B from 11-14 minutes. The flow rate of the system was set at 0.35 mL/min, and the column temperature was held constant at 40°C, with an injection volume of 4 μL. Multiple reaction monitoring (MRM) mode was used to acquire data from the production scan, which was then analyzed with the Metabolites Database (METWARE database) for metabolite identification. Quantitative analysis of metabolites was then performed using Analyst 1.6.3 software, with further examination of the metabolic pathways of these compounds using the KEGG database ( http://www.kegg.jp). Transcriptome analysis Winkel-Shirley, B. (2001). Flavonoid biosynthesis. a colorful model for genetics, biochemistry, cell biology, and biotechnology. Plant Physiol. 126, 485–493. doi:10.1104/pp.126.2.485 Citation: Xiong B, Li Q, Yao J, Liu Z, Yang X, Yu X, Li Y, Liao L, Wang X, Deng H, Zhang M, Sun G and Wang Z (2023) Widely targeted metabolomic profiling combined with transcriptome analysis sheds light on flavonoid biosynthesis in sweet orange 'Newhall' (C. sinensis) under magnesium stress. Front. Plant Sci. 14:1182284. doi: 10.3389/fpls.2023.1182284 Li, C. P., Qi, Y. P., Zhang, J., Yang, L. T., Wang, D. H., Ye, X., et al. (2017). Magnesium-deficiency-induced alterations of gas exchange, major metabolites and key enzymes differ among roots, and lower and upper leaves of citrus sinensis seedlings. Tree Physiol. 37, 1564–1581. doi:10.1093/treephys/tpx067
Ferrer, J. L., Austin, M. B., Stewart, C., Jr., Noel, J. P. (2008). Structure and function of enzymes involved in the biosynthesis of phenylpropanoids. Plant Physiol. Biochem. 46, 356–370. doi:10.1016/j.plaphy.2007.12.009 Treutter, D. (2005). Significance of flavonoids in plant resistance and enhancement of their biosynthesis. Plant Biol. (Stuttg) 7, 581–591. doi:10.1055/s-2005-873009 Long, A., Zhang, J., Yang, L. T., Ye, X., Lai, N. W., Tan, L. L., et al. (2017). Effects of low pH on photosynthesis, related physiological parameters, and nutrient profiles of citrus. Front. Plant Sci. 8. doi:10.3389/fpls.2017.00185 Zhang, B., Yang, H. J., Qu, D., Zhu, Z. Z., Yang, Y. Z., Zhao, Z. Y. (2022). The MdBBX22-miR858-MdMYB9/11/12 module regulates proanthocyanidin biosynthesis in apple peel. Plant Biotechnol. J. 20, 1683–1700. doi:10.1111/pbi.13839 Saito, K., Yonekura-Sakakibara, K., Nakabayashi, R., Higashi, Y., Yamazaki, M., Tohge, T., et al. (2013). The flavonoid biosynthetic pathway in arabidopsis: structural and genetic diversity. Plant Physiol. Biochem. 72, 21–34. doi:10.1016/j.plaphy.2013.02.001